whole human genome oligo microarrays 4 × 44k  (Agilent technologies)

Journal: Molecular Vision

Article Title: Genome-wide changes accompanying the knockdown of Ep-CAM in retinoblastoma

doi:

Figure Lengend Snippet: Upregulated genes identified by microarray post EpCAM knock-down in Y79

Article Snippet: Qiagen’s RNeasy mini spin columns were used for purification of the amplified cRNA samples, and the samples were then hybridized to the Human Whole Genome 44K Oligo Microarray for 17 h at 65 °C, as recommended by the manufacturer (Agilent Technologies).

Techniques: Microarray, Cell Differentiation, Variant Assay, Binding Assay

Journal: Molecular Vision

Article Title: Genome-wide changes accompanying the knockdown of Ep-CAM in retinoblastoma

doi:

Figure Lengend Snippet: Down-regulated genes identified by microarray post EpCAM Knock-down in Y79

Article Snippet: Qiagen’s RNeasy mini spin columns were used for purification of the amplified cRNA samples, and the samples were then hybridized to the Human Whole Genome 44K Oligo Microarray for 17 h at 65 °C, as recommended by the manufacturer (Agilent Technologies).

Techniques: Microarray, Variant Assay, Binding Assay, Activation Assay, Amplification, Sequencing, Derivative Assay, RNA Binding Assay

Journal: Molecular Vision

Article Title: Genome-wide changes accompanying the knockdown of Ep-CAM in retinoblastoma

doi:

Figure Lengend Snippet: The mRNA expression of selected genes from microarray data was confirmed using real time quantitative reverse transcriptase PCR (Q-RT–PCR). The black bars represent the mRNA levels quantified by Q-RT–PCR and the white bars represent the fold expression of genes studied by microarray. The Q-RT–PCR results are consistent with microarray results. The error bars represent the standard deviation of triplicate values. Abbreviations: PCNA represents proliferating cell nuclear antigen, CCDN3 represents cyclin D3, FOS represents v-fos FBJ murine osteosarcoma viral oncogene homolog, JUN represents jun oncogene, and DRAM represents damage-regulated autophagy modulator.

Article Snippet: Qiagen’s RNeasy mini spin columns were used for purification of the amplified cRNA samples, and the samples were then hybridized to the Human Whole Genome 44K Oligo Microarray for 17 h at 65 °C, as recommended by the manufacturer (Agilent Technologies).

Techniques: Expressing, Microarray, Reverse Transcription Polymerase Chain Reaction, Standard Deviation

Journal: PLoS ONE

Article Title: PTCH1 +/− Dermal Fibroblasts Isolated from Healthy Skin of Gorlin Syndrome Patients Exhibit Features of Carcinoma Associated Fibroblasts

doi: 10.1371/journal.pone.0004818

Figure Lengend Snippet: RT-QPCR validation of microarray results/Relative level of up and down-regulated mRNA in control and NBCCS fibroblasts * .

Article Snippet: Hybridizations were carried out for 17 hours at 60°C with 1 µg of purified control and NBCCS probes on Agilent ® human whole genome oligo microarray 44k.

Techniques: Microarray

Journal: PLoS ONE

Article Title: PTCH1 +/− Dermal Fibroblasts Isolated from Healthy Skin of Gorlin Syndrome Patients Exhibit Features of Carcinoma Associated Fibroblasts

doi: 10.1371/journal.pone.0004818

Figure Lengend Snippet: The levels of secreted MMP1, MMP3 and FGF7 were determined by ELISA in the supernatants of the dermal equivalents used for the microarray assay. Results were set to 1 for the protein levels in the supernatant of the dermal equivalent with control (CTRL) fibroblast strain 1. The protein level in control strains is represented with grey histogram and with black histogram in NBCCS strains. The average MMPs and FGF7 levels in the supernatants of control (NBCCS) dermal equivalents are indicated with a plain (dotted) line. The ratios between the average protein levels in NBCCS and in control fibroblasts are indicated (arrow).

Article Snippet: Hybridizations were carried out for 17 hours at 60°C with 1 µg of purified control and NBCCS probes on Agilent ® human whole genome oligo microarray 44k.

Techniques: Enzyme-linked Immunosorbent Assay, Microarray